Scalable high-density peptide arrays for comprehensive health monitoring

Nat Commun. 2014 Sep 3;5:4785. doi: 10.1038/ncomms5785.

Abstract

There is an increasing awareness that health care must move from post-symptomatic treatment to presymptomatic intervention. An ideal system would allow regular inexpensive monitoring of health status using circulating antibodies to report on health fluctuations. Recently, we demonstrated that peptide microarrays can do this through antibody signatures (immunosignatures). Unfortunately, printed microarrays are not scalable. Here we demonstrate a platform based on fabricating microarrays (~10 M peptides per slide, 330,000 peptides per assay) on silicon wafers using equipment common to semiconductor manufacturing. The potential of these microarrays for comprehensive health monitoring is verified through the simultaneous detection and classification of six different infectious diseases and six different cancers. Besides diagnostics, these high-density peptide chips have numerous other applications both in health care and elsewhere.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / blood*
  • Antibody Specificity
  • Communicable Diseases / blood*
  • Communicable Diseases / diagnosis
  • Communicable Diseases / immunology
  • Health Status
  • Humans
  • Lab-On-A-Chip Devices*
  • Microtechnology / methods
  • Molecular Sequence Data
  • Monitoring, Physiologic / instrumentation
  • Monitoring, Physiologic / methods
  • Neoplasms / blood*
  • Neoplasms / diagnosis
  • Neoplasms / immunology
  • Peptides / chemical synthesis
  • Peptides / immunology
  • Protein Array Analysis / instrumentation*
  • Protein Array Analysis / methods
  • Quality Control
  • Silicon / chemistry

Substances

  • Antibodies, Monoclonal
  • Peptides
  • Silicon