A CRISPR view of development

Genes Dev. 2014 Sep 1;28(17):1859-72. doi: 10.1101/gad.248252.114.

Abstract

The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is poised to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism. This RNA-guided nuclease (RGN)-based approach already has been effectively used to induce targeted mutations in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins. Illustrating the adaptability of RGNs, the genomes of >20 different plant and animal species as well as multiple cell lines and primary cells have been successfully modified. Here we review the current and potential uses of RGNs to investigate genome function during development.

Keywords: CRISPR; Cas9; RNA-guided nuclease; development; genome editing; genome engineering.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • CRISPR-Associated Proteins / metabolism*
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
  • Gene Expression Regulation, Developmental*
  • Genome / genetics*
  • Humans
  • Mutation / genetics
  • RNA Editing / genetics
  • RNA, Guide / metabolism

Substances

  • CRISPR-Associated Proteins
  • RNA, Guide