Active hexose correlated compound exerts therapeutic effects in lymphocyte driven colitis

Mol Nutr Food Res. 2014 Dec;58(12):2379-82. doi: 10.1002/mnfr.201400364. Epub 2014 Oct 2.


Active hexose correlated compound (AHCC) is a commercial extract of Basidiomycetes fungi enriched in oligosaccharides that is used as a human nutritional supplement for various purposes in humans. Our aim was to study the anti-inflammatory effect of AHCC in the CD4+ CD62L(+) T cell transfer model of colitis, considered one of the closest to the human disease. Colitis was induced by transfer of CD4(+) CD62L(+) T cells to recombination activating gene 1(-/-) mice. AHCC (75 mg/d) was administered by gavage as a post-treatment. Three groups were established: noncolitic, colitic (CD4(+) CD62L(+) transferred mice treated with vehicle), and AHCC (colitic treated with AHCC). AHCC improved colitis, as evidenced by a 24% lower colonic myeloperoxidase and a 21% lower alkaline phosphatase activity. In addition, a decreased secretion of proinflammatory genes assessed by RT-qPCR was observed, particularly TNF-α and IL-1β. Ex vivo mesenteric lymph node cells obtained from AHCC treated mice exhibited a fully normalized production of IL-6, IL-17, and IL-10 (p < 0.05). Also, AHCC treated mice exhibited decreased STAT4 and IκB-α phosphorylation in splenic CD4(+) cells. Our data provide validation of AHCC colonic anti-inflammatory activity in a chronic, T cell driven model of inflammatory bowel disease.

Keywords: AHCC; CD4+ CD62L+ T cell; Inflammatory bowel disease; Nutraceutical; Prebiotic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • CD4-Positive T-Lymphocytes / drug effects*
  • CD4-Positive T-Lymphocytes / metabolism
  • Colitis / drug therapy*
  • Colon / drug effects
  • Colon / pathology
  • Disease Models, Animal
  • I-kappa B Proteins / genetics
  • I-kappa B Proteins / metabolism
  • Interleukin-10 / metabolism
  • Interleukin-17 / metabolism
  • Interleukin-1beta / genetics
  • Interleukin-1beta / metabolism
  • Interleukin-6 / metabolism
  • Mice
  • NF-KappaB Inhibitor alpha
  • Phosphorylation
  • Polysaccharides / pharmacology*
  • Reproducibility of Results
  • STAT4 Transcription Factor / genetics
  • STAT4 Transcription Factor / metabolism
  • Spleen / cytology
  • Spleen / drug effects
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism


  • Anti-Inflammatory Agents
  • I-kappa B Proteins
  • Interleukin-17
  • Interleukin-1beta
  • Interleukin-6
  • NFKBIA protein, human
  • Nfkbia protein, mouse
  • Polysaccharides
  • STAT4 Transcription Factor
  • Stat4 protein, mouse
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • NF-KappaB Inhibitor alpha
  • Active Hexose Correlated Compound