Generation of Cerebral Organoids From Human Pluripotent Stem Cells

Nat Protoc. 2014 Oct;9(10):2329-40. doi: 10.1038/nprot.2014.158. Epub 2014 Sep 4.

Abstract

Human brain development exhibits several unique aspects, such as increased complexity and expansion of neuronal output, that have proven difficult to study in model organisms. As a result, in vitro approaches to model human brain development and disease are an intense area of research. Here we describe a recently established protocol for generating 3D brain tissue, so-called cerebral organoids, which closely mimics the endogenous developmental program. This method can easily be implemented in a standard tissue culture room and can give rise to developing cerebral cortex, ventral telencephalon, choroid plexus and retinal identities, among others, within 1-2 months. This straightforward protocol can be applied to developmental studies, as well as to the study of a variety of human brain diseases. Furthermore, as organoids can be maintained for more than 1 year in long-term culture, they also have the potential to model later events such as neuronal maturation and survival.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain / cytology
  • Brain / growth & development*
  • Humans
  • Neuroepithelial Cells / physiology
  • Organoids / growth & development*
  • Pluripotent Stem Cells*
  • Time Factors
  • Tissue Culture Techniques / instrumentation
  • Tissue Culture Techniques / methods*