FOXP3+ lymphocyte density in pancreatic cancer correlates with lymph node metastasis

PLoS One. 2014 Sep 5;9(9):e106741. doi: 10.1371/journal.pone.0106741. eCollection 2014.

Abstract

Objective: To determine if the density of FOXP3+ lymphocytes in primary tumors and lymph nodes in pancreatic cancer correlates with the presence of lymph node metastases.

Methods: FOXP3+ lymphocyte density in primary pancreatic cancer tissue and draining lymph nodes was measured using immunohistochemistry. We analyzed the clinical and pathological aspects associated with the accumulation of FOXP3+ lymphocytes in pancreatic cancer. We also analyzed the correlation of density of FOXP3+ lymphocytes in lymph nodes with the nodal status and distance from the primary tumor.

Results: FOXP3+ lymphocyte density in pancreatic cancer was significantly higher than in paratumoral pancreatic tissue. The density of FOXP3+ lymphocytes in local tumor tissue correlated significantly with the histological grade and overall lymph node status. Furthermore, FOXP3+ lymphocyte density was significantly higher in positive lymph nodes than in negative ones, while it had no correlation with the distance of the lymph node from the primary tumor.

Conclusion: FOXP3+ lymphocyte density in primary tumor tissue in patients with pancreatic cancer correlates with lymph node metastasis. Lymph nodes containing metastases having higher FOXP3+ lymphocyte densities than do negative lymph nodes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Cell Count
  • Female
  • Forkhead Transcription Factors / metabolism*
  • Humans
  • Lymph Nodes / immunology
  • Lymph Nodes / metabolism
  • Lymph Nodes / pathology*
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Pancreatic Neoplasms / immunology*
  • Pancreatic Neoplasms / pathology*
  • Prognosis
  • T-Lymphocytes, Regulatory / cytology*
  • T-Lymphocytes, Regulatory / metabolism
  • Young Adult

Substances

  • FOXP3 protein, human
  • Forkhead Transcription Factors

Grant support

This study was supported by grants from Shanghai Municipal Natural Science Foundation (11ZR1405500) and grants from Shanghai Municipal Science and Technology Commission (13140902401). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.