[Heterotransplantability of human anaplastic thyroid carcinoma line (HOTHC) in nude mice, and biological properties of the heterograft]

Hum Cell. 1989 Mar;2(1):74-9.
[Article in Japanese]

Abstract

The heterotransplantability of HOTHC line (human anaplastic thyroid carcinoma) into the subcutis of BALB/c nude mice and the biological properties of grafts were discussed. (1) The HOTHC line showed high transplantability, and 1 x 10(4) cells produced anaplastic carcinoma (giant cell type) containing the colloid-like substance. (2) The grafted tumors grew rapidly and the mice were dead within 2 months after transplantation. (3) The number of leukocytes (neutrophils) of mouse peripheral blood increased as the tumor size increased, and the leukocyte count returned to a normal value after removal of the tumor. (4) The conditioned media of HOTHC line formed colonies of granulocytes (neutrophils) on soft agar. These phenomena revealed that HOTHC is a granulocyte-colony stimulating factor-producing line. (5) The conditioned media of HOTHC line showed promoting-effect on neovascularization on the chorioallantoic membrane.

Publication types

  • English Abstract

MeSH terms

  • Aged
  • Aged, 80 and over
  • Angiogenesis Inducing Agents / metabolism
  • Animals
  • Carcinoma / immunology
  • Carcinoma / metabolism
  • Carcinoma / pathology*
  • Cell Division
  • Female
  • Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
  • Humans
  • Leukocyte Count
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation*
  • Thyroid Neoplasms / immunology
  • Thyroid Neoplasms / metabolism
  • Thyroid Neoplasms / pathology*
  • Transplantation, Heterologous*
  • Tumor Cells, Cultured

Substances

  • Angiogenesis Inducing Agents
  • Granulocyte-Macrophage Colony-Stimulating Factor