Effect of hesperidin incorporation into a self-etching primer on durability of dentin bond

Dent Mater. 2014 Nov;30(11):1205-12. doi: 10.1016/j.dental.2014.08.371. Epub 2014 Sep 5.


Objective: Collagen degradation at the resin-dentin interface deteriorates dentin bond durability. The use of natural cross-linkers might offer a positive approach to stabilize the resin-dentin interface. This study evaluated the effects of incorporation of natural cross-linkers into a self-etch adhesive primer on the immediate and long-term micro-tensile bond strengths (μTBS) to dentin.

Methods: Experimental primers were prepared by incorporating either 0.5%, 1%, 2%, 5% of hesperidin (HPN) or 0.5% of proanthocyanidins (PA) into Clearfil SE primer. Extracted human molar teeth were restored using the experimental primers or the pure SE primer (control). The mechanical properties of the bonded interfaces were measured using the nano-indentation tests. Beam-shaped bonded specimens were sub-divided for one-day and one-year μTBS test. Interfacial collagen morphology was observed using transmission electron microscopy.

Result: The immediate μTBS significantly increased in 0.5%, 1% and 2% HPN-incorporated groups when compared with the control. The mechanical properties of bonded interface were improved with 1% and 2% HPN-incorporated primers. For the long-term μTBS, the 2% and 5% HPN-incorporated group were significantly higher than the control. The morphology of the collagen fibrils were preserved by 5% HPN-incorporation after one-year storage. The PA group, however, failed to improve the μTBS and the mechanical properties of the bonded interfaces.

Significance: The incorporation of 2% HPN into the self-etching primer had a positive effect on the immediate μTBS and mechanical properties of the resin-dentin interfaces. The 5% HPN group preserved the morphology of the collagen in the hybrid layer after one-year storage in artificial saliva.

Keywords: Collagen; Cross-linker; Elastic modulus; Hardness; Hesperidin; Hybrid layer; Micro-tensile bond strength.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Etching, Dental*
  • Dentin / metabolism*
  • Hesperidin / metabolism*
  • Microscopy, Electron, Transmission
  • Tensile Strength


  • Hesperidin