Mixtures of Opposing Phosphorylations Within Hexamers Precisely Time Feedback in the Cyanobacterial Circadian Clock

Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):E3937-45. doi: 10.1073/pnas.1408692111. Epub 2014 Sep 2.


Circadian oscillations are generated by the purified cyanobacterial clock proteins, KaiA, KaiB, and KaiC, through rhythmic interactions that depend on multisite phosphorylation of KaiC. However, the mechanisms that allow these phosphorylation reactions to robustly control the timing of oscillations over a range of protein stoichiometries are not clear. We show that when KaiC hexamers consist of a mixture of differentially phosphorylated subunits, the two phosphorylation sites have opposing effects on the ability of each hexamer to bind to the negative regulator KaiB. We likewise show that the ability of the positive regulator KaiA to act on KaiC depends on the phosphorylation state of the hexamer and that KaiA and KaiB recognize alternative allosteric states of the KaiC ring. Using mathematical models with kinetic parameters taken from experimental data, we find that antagonism of the two KaiC phosphorylation sites generates an ultrasensitive switch in negative feedback strength necessary for stable circadian oscillations over a range of component concentrations. Similar strategies based on opposing modifications may be used to support robustness in other timing systems and in cellular signaling more generally.

Keywords: allostery; circadian rhythms; mathematical modeling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allosteric Regulation
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism*
  • Circadian Clocks*
  • Circadian Rhythm
  • Cyanobacteria / physiology*
  • Feedback, Physiological*
  • Models, Biological
  • Phosphorylation
  • Phosphoserine / metabolism
  • Protein Binding
  • Protein Multimerization*
  • Protein Stability
  • Protein Subunits / metabolism
  • Time Factors


  • Bacterial Proteins
  • Protein Subunits
  • Phosphoserine