Cytokine gene polymorphisms are associated with susceptibility to tuberculosis in an Amerindian population

Int J Tuberc Lung Dis. 2014 Aug;18(8):952-7. doi: 10.5588/ijtld.14.0060.


Setting: Cytokines play an important role in anti-tuberculosis immune response, combined with antigen-presenting cells and lymphocytes. Immune response gene polymorphisms have been reported to be associated with tuberculosis (TB) susceptibility in some but not all studies.

Objective: To evaluate the association of immune response genes with susceptibility to tuberculin skin test (TST) reactivity and/or TB.

Design: Fourteen single nucleotide polymorphisms were genotyped in 96 individuals of the Aché, a native Paraguayan population, by allelic discrimination using real-time polymerase chain reaction. Univariate and multivariate Poisson regression were employed to assess risk genotypes.

Results: A higher prevalence of purified protein derivative reactivity was associated with the TNF-α CCA/TCG haplotype (PR 1.298, 95%CI 1.059-1.589) and with the IL-10 AT/CC diplotype (PR 1.181, 95% CI 1.024-1.362), and the presence of the IL-8 rs4073 T allele was associated with protection against TB (PR 0.482, 95%CI 0.273-0.851).

Conclusions: These results suggest that polymorphisms in genes associated with immune response are involved in TST reactivity and susceptibility to TB in the Aché population.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Case-Control Studies
  • Female
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Indians, South American / genetics
  • Interleukin-10 / genetics*
  • Interleukin-8 / genetics*
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Paraguay
  • Poisson Distribution
  • Polymorphism, Single Nucleotide
  • Real-Time Polymerase Chain Reaction
  • Regression Analysis
  • Tuberculin Test
  • Tuberculosis / epidemiology*
  • Tuberculosis / genetics
  • Tuberculosis / immunology
  • Tumor Necrosis Factor-alpha / genetics*
  • Young Adult


  • Interleukin-8
  • Tumor Necrosis Factor-alpha
  • Interleukin-10