IL-6 Trans-Signaling-Dependent Rapid Development of Cytotoxic CD8+ T Cell Function

Cell Rep. 2014 Sep 11;8(5):1318-27. doi: 10.1016/j.celrep.2014.07.008. Epub 2014 Sep 4.

Abstract

Immune control of infections with viruses or intracellular bacteria relies on cytotoxic CD8(+) T cells that use granzyme B (GzmB) for elimination of infected cells. During inflammation, mature antigen-presenting dendritic cells instruct naive T cells within lymphoid organs to develop into effector T cells. Here, we report a mechanistically distinct and more rapid process of effector T cell development occurring within 18 hr. Such rapid acquisition of effector T cell function occurred through cross-presenting liver sinusoidal endothelial cells (LSECs) in the absence of innate immune stimulation and known costimulatory signaling. Rather, interleukin-6 (IL-6) trans-signaling was required and sufficient for rapid induction of GzmB expression in CD8(+) T cells. Such LSEC-stimulated GzmB-expressing CD8(+) T cells further responded to inflammatory cytokines, eliciting increased and protracted effector functions. Our findings identify a role for IL-6 trans-signaling in rapid generation of effector function in CD8(+) T cells that may be beneficial for vaccination strategies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism
  • Cell Line
  • Cells, Cultured
  • Cytotoxicity, Immunologic*
  • Granzymes / genetics
  • Granzymes / metabolism
  • Interleukin-6 / metabolism*
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Signal Transduction*

Substances

  • Interleukin-6
  • Granzymes