Metabolomics of AS-5 RBC supernatants following routine storage

Vox Sang. 2015 Feb;108(2):131-40. doi: 10.1111/vox.12193. Epub 2014 Sep 9.


Background and objectives: The safety and efficacy of stored red blood cells (RBCs) transfusion has been long debated due to retrospective clinical evidence and laboratory results, indicating a potential correlation between increased morbidity and mortality following transfusion of RBC units stored longer than 14 days. We hypothesize that storage in Optisol additive solution-5 leads to a unique metabolomics profile in the supernatant of stored RBCs.

Materials and methods: Whole blood was drawn from five healthy donors, RBC units were manufactured, and prestorage leucoreduced by filtration. Samples were taken on days 1 and 42, the cells removed, and mass spectrometry-based metabolomics was performed.

Results: The results confirmed the progressive impairment of RBC energy metabolism by day 42 with indirect markers of a parallel alteration of glutathione and NADPH homeostasis. Moreover, oxidized pro-inflammatory lipids accumulated by the end of storage.

Conclusion: The supernatants from stored RBCs may represent a burden to the transfused recipients from a metabolomics standpoint.

Keywords: oxidative stress; red blood cell; storage; transfusion medicine.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Blood Preservation / methods*
  • Erythrocyte Transfusion / methods
  • Erythrocytes / metabolism*
  • Humans
  • Mass Spectrometry
  • Metabolome*
  • Retrospective Studies