A new ELISA for quantification of brassinosteroids in plants

Steroids. 2015 May:97:78-86. doi: 10.1016/j.steroids.2014.08.022. Epub 2014 Sep 6.

Abstract

Starting from (22R,23R)-2α,3α,22,23,26-pentahydroxy-5α-cholestan-6-one 26-hemisuccinate, conjugates of 28-norcastasterone with horse radish peroxidase and bovine serum albumin were prepared. The latter conjugate was injected into rabbits; produced polyclonal antibodies were used to quantitate 6-keto-brassinosteroids. The newly developed analytical system was used in combination with two other immunoenzymatic assays for brassinosteroids to determine individual compounds of this series. In addition, a direct method of brassinosteroid analysis was proposed. It has the advantage of requiring no sample pretreatment steps such as extraction with organic solvents and chromatography.

Keywords: Brassinosteroids; Flores Chamomillae; HPLC; Phytohormones; Synthesis; ЕLISA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brassinosteroids / analysis*
  • Cattle
  • Chamomile / chemistry*
  • Enzyme-Linked Immunosorbent Assay*
  • Horseradish Peroxidase / chemistry
  • Horseradish Peroxidase / metabolism
  • Horses
  • Molecular Conformation
  • Rabbits
  • Serum Albumin, Bovine / chemistry
  • Serum Albumin, Bovine / metabolism

Substances

  • Brassinosteroids
  • Serum Albumin, Bovine
  • Horseradish Peroxidase