Development of a novel in vivo cancer model using cell sheet engineering

Anticancer Res. 2014 Sep;34(9):4747-54.


Aim: Standard in vivo cancer models entail injecting single cancer cells, but this technique is not always successful. We developed a novel cancer cell sheet by using temperature-responsive polymer poly(N-isopropyl acryl amide)-coated plates, which allow controlled attachment and detachment of living cancer cells via simple temperature changes.

Materials and methods: Four human cancer cell lines were used to make cell sheets. The cancer cell sheets were subcutaneously transplanted into nude mice and compared regarding their tumor-forming ability with the conventional cell suspension technique.

Results: Human cancer cell sheets were successfully transplanted into nude mice. The cancer cell sheets resulted in stable engraftment and showed a higher tumor volume determined by total flux with the IVIS® imaging system.

Conclusion: Novel cancer cell sheets are useful tools to make in vivo cancer models in mice for the assessment of anticancer therapeutics.

Keywords: Cancer; cell sheet; mouse model; tissue engineering; transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques*
  • Cell Line, Tumor
  • Cell Proliferation
  • Female
  • HCT116 Cells
  • Humans
  • Mice
  • Neoplasms / pathology
  • Tumor Cells, Cultured
  • Xenograft Model Antitumor Assays