Elucidation of some fragmentations of small peptides using sequential mass spectrometry on a hybrid instrument

Rapid Commun Mass Spectrom. 1989 Jul;3(7):217-21. doi: 10.1002/rcm.1290030704.


Sequential daughter-ion-scanning analyses of small peptides have been performed using a hybrid tandem instrument of BEqQ configuration. Precursor ions are selected by B and allowed or induced (by high-energy collisional activation) to decompose in the region preceding E. Decoupling of E from the accelerating voltage permits the selection of the first-generation daughter ion whilst retaining appropriate float voltages for the quadrupole assemblies. The daughter ion selected by E is further subjected to low-energy collisional-activation dissociation (CAD) in q and the fragment-ion spectrum is obtained by scanning Q. The sequential daughter-ion-scanning technique has been used to establish that 'internal' fragments of the types, (AY') and (BY'), are formed via initial Y-type cleavage. Fragmentation of a protonated peptide (angiotensin III) by loss of the C-terminal amino acid residue, yielding a (B'n + OH) ion, is reported for the first time. This process is analogous to that previously described for metal-cationized peptides.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Angiotensin II / chemistry
  • Angiotensin III / chemistry
  • Enkephalin, Leucine / chemistry
  • Enkephalin, Methionine / chemistry
  • Mass Spectrometry / instrumentation
  • Mass Spectrometry / methods
  • Peptide Fragments / chemistry*


  • Peptide Fragments
  • Angiotensin II
  • Angiotensin III
  • Enkephalin, Methionine
  • Enkephalin, Leucine