The water samples of the Tien Lake were collected for the three-dimensional fluorescence spectrum detected. And parallel factor analysis (PARAFAC) and principal component analysis (PCA) were used for the spectra analyzing to know the main factor and relative contribution of the chromophoric dissolved organic matter (CDOM). PARAFAC decomposed CDOM into four components, the humic-like: C1 (240, 415), C3 (265, 525), C4 (255, 505) and the protein-like: C2(230/280, 330). The fluorescence intensity of CDOM components was high in north and estuary of the lake, but low in the other region. All the four components show significant positive correlations (p < 0.01), this result means they may have the same sources. Principal component analysis shows that these four components of the Tien Lake are all from terrestrial organic matter, meanwhile its DTN, DTP, DON may also come with the terrestrial substance. These components of Tien Lake can well be connected with dissolved nutrient salts by nonlinear multiple regression, which means we can use the three-dimensional fluorescence spectrum results of CDOM to indicate the eutrophication degree of Tien Lake.