Characterization of swine leucocyte antigen alleles in a crossbred pig to be used in xenotransplant studies

Tissue Antigens. 2014 Nov;84(5):484-8. doi: 10.1111/tan.12430. Epub 2014 Sep 11.

Abstract

We have characterized swine leucocyte antigen (SLA) classes I and II molecules of a domestic pig as a model for use in our xenotransplant program. Molecular characterization of the SLA classes I and II genes is critical to understanding the adaptive immune responses between swine and humans in the event of xenotransplantation. Seven swine leucocyte antigen genes (SLA-1, SLA-2, SLA-3, DQB1, DRB1, DQA and DRA) were analyzed and 15 alleles were identified. A novel DRA*w04re01 is reported for this limited polymorphic class II gene. The heterozygous haplotypes, Hp-32.0/35.0 and Hp-0.13/0.23 were deduced for our IU-pig model, for SLA classes I and II regions, respectively.

Keywords: Sus scrofa; genotyping; haplotypes; polymorphism; swine leucocyte antigen; xenotransplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptive Immunity
  • Alleles*
  • Animals
  • Crosses, Genetic
  • Haplotypes*
  • Heterozygote*
  • Histocompatibility Antigens Class I / genetics*
  • Histocompatibility Antigens Class I / immunology
  • Histocompatibility Antigens Class II / genetics*
  • Histocompatibility Antigens Class II / immunology
  • Swine / genetics*
  • Swine / immunology

Substances

  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II