Unique antibody responses to malondialdehyde-acetaldehyde (MAA)-protein adducts predict coronary artery disease

PLoS One. 2014 Sep 11;9(9):e107440. doi: 10.1371/journal.pone.0107440. eCollection 2014.

Abstract

Malondialdehyde-acetaldehyde adducts (MAA) have been implicated in atherosclerosis. The purpose of this study was to investigate the role of MAA in atherosclerotic disease. Serum samples from controls (n = 82) and patients with; non-obstructive coronary artery disease (CAD), (n = 40), acute myocardial infarction (AMI) (n = 42), or coronary artery bypass graft (CABG) surgery due to obstructive multi-vessel CAD (n = 72), were collected and tested for antibody isotypes to MAA-modifed human serum albumin (MAA-HSA). CAD patients had elevated relative levels of IgG and IgA anti-MAA, compared to control patients (p<0.001). AMI patients had a significantly increased relative levels of circulating IgG anti-MAA-HSA antibodies as compared to stable angina (p<0.03) or CABG patients (p<0.003). CABG patients had significantly increased relative levels of circulating IgA anti-MAA-HSA antibodies as compared to non-obstructive CAD (p<0.001) and AMI patients (p<0.001). Additionally, MAA-modified proteins were detected in the tissue of human AMI lesions. In conclusion, the IgM, IgG and IgA anti-MAA-HSA antibody isotypes are differentially and significantly associated with non-obstructive CAD, AMI, or obstructive multi-vessel CAD and may serve as biomarkers of atherosclerotic disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetaldehyde / immunology*
  • Aged
  • Autoantibodies / blood*
  • Biomarkers / blood
  • Case-Control Studies
  • Coronary Artery Disease / blood
  • Coronary Artery Disease / diagnosis
  • Coronary Artery Disease / immunology*
  • Female
  • Humans
  • Lipoproteins, LDL / immunology*
  • Male
  • Malondialdehyde / immunology*
  • Middle Aged
  • Plaque, Atherosclerotic / blood
  • Plaque, Atherosclerotic / immunology

Substances

  • Autoantibodies
  • Biomarkers
  • Lipoproteins, LDL
  • Malondialdehyde
  • Acetaldehyde

Grants and funding

This work was funded by the NIH Student Loan Repayment Program (DRA), the Division of Cardiology, and Department of Internal Medicine at the University of Nebraska Medical Center, Omaha, Nebraska. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.