Anti-inflammatory activities of tormentic acid from suspension cells of Eriobotrya Japonicaex vivo and in vivo

Chwen-Tzuei Chang; Shyh-Shyun Huang; Shiang-Shiou Lin; Sakae Amagaya; Hui-Ya Ho; Wen-Chi Hou; Pei-Hsin Shie; Jin-Bin Wu; Guan-Jhong Huang

doi:10.1016/j.foodchem.2011.01.114

Anti-inflammatory activities of tormentic acid from suspension cells of Eriobotrya Japonicaex vivo and in vivo

Food Chem. 2011 Aug 1;127(3):1131-7. doi: 10.1016/j.foodchem.2011.01.114. Epub 2011 Jan 31.

Authors

Chwen-Tzuei Chang¹, Shyh-Shyun Huang², Shiang-Shiou Lin², Sakae Amagaya³, Hui-Ya Ho⁴, Wen-Chi Hou⁵, Pei-Hsin Shie², Jin-Bin Wu⁶, Guan-Jhong Huang⁷

Affiliations

¹ Department of Internal Medicine, Division of Endocrinology and Metabolism, China Medical University Hospital, Taichung, Taiwan.
² School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan.
³ Department of Kampo Pharmaceutical Sciences, Nihon Pharmaceutical University, Japan.
⁴ Jen Li Biotech Co. Ltd., Yong-feng Road, Taiping City, Taichung 411, Taiwan.
⁵ Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan.
⁶ Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung, Taiwan. Electronic address: jinbinwu@gmail.com.
⁷ School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan. Electronic address: gjhuang@mail.cmu.edu.tw.

PMID: 25214105
DOI: 10.1016/j.foodchem.2011.01.114

Abstract

Anti-inflammatory effects of tormentic acid (TA) were investigated ex vivo and in vivo. TA decreased the paw edema at the 4th and 5thhour after λ-carrageenin (Carr) administration, and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. TA also significantly attenuated the thiobarbituric acid reactive substances (TBARS) level in the edematous paw at the 5thhour after Carr injection. TA decreased the nitric oxide (NO) levels on the serum level and diminished the serum tumour necrosis factor (TNF-α) at the 5thhour after Carr injection. Western blotting revealed that the TA decreased Carr-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions. As per results, the anti-inflammatory mechanisms of TA might be correlated to the decrease in the level of TBARS, iNOS, and COX-2 in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver.

Keywords: Anti-inflammation; NO; TBARS; TNF-α; Tormentic acid.