Antioxidant and cholinesterases inhibitory activities of Verbascum xanthophoeniceum Griseb. and its phenylethanoid glycosides

Milen Georgiev; Kalina Alipieva; Ilkay Orhan; Radoslav Abrashev; Petko Denev; Maria Angelova

doi:10.1016/j.foodchem.2011.02.083

Antioxidant and cholinesterases inhibitory activities of Verbascum xanthophoeniceum Griseb. and its phenylethanoid glycosides

Food Chem. 2011 Sep 1;128(1):100-5. doi: 10.1016/j.foodchem.2011.02.083. Epub 2011 Mar 2.

Authors

Milen Georgiev¹, Kalina Alipieva², Ilkay Orhan³, Radoslav Abrashev⁴, Petko Denev⁵, Maria Angelova⁴

Affiliations

¹ Department of Microbial Biosynthesis and Biotechnologies, Laboratory in Plovdiv, Institute of Microbiology, Bulgarian Academy of Sciences, Bulgaria; Division of Pharmacognosy, Section Metabolomics, Institute of Biology, Leiden University, Leiden, The Netherlands. Electronic address: Milen.Georgiev@mailbox.tu-dresden.de.
² Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria.
³ Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
⁴ Department of Mycology, Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
⁵ Laboratory of Bioactive Substances, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Plovdiv, Bulgaria.

PMID: 25214335
DOI: 10.1016/j.foodchem.2011.02.083

Abstract

The members of Verbascum L. (Scrophulariaceae) are known to be rich in phenylethnoid glycosides, and among them Verbascum xanthophoeniceum is an endemic plant species for the Balkan region, Northwestern, and Southern Turkey. A scheme was developed for the isolation of the main active constituents that accumulate in plant aerial parts. The antioxidant activities of total methanol extracts, collected phenylethanoid glycosides fractions and specific active constituents (forsythoside B, verbascoside and leucosceptoside B) were then evaluated in 2,2'-diphenyl-1-picrylhydrazyl (DPPH·), oxygen radical absorbance capacity (ORACFL), hydroxyl radical averting capacity (HORACFL), ferric-reducing antioxidant power (FRAP), and superoxide anion (O2(-)) radical scavenging assays. In vitro acetylcholinesterase (AChE) and butyrylcholinesterase (BChe) inhibitory activities of abovementioned extracts, fractions and isolated pure compounds were also examined. Depending on the method used, forsythoside B, verbascoside and leucosceptoside B proved to be effective radical scavengers and cholinesterases inhibitors. On the basis of these findings it can be proposed that in addition to providing a potent source of antimicrobial and anti-inflammatory compounds, Verbascum plants could serve as attractive mines of powerful antioxidants for various purposes.

Keywords: AChE and BChE inhibitions; DPPH; FRAP; HORAC(FL); ORAC(FL); Phenylethanoid glycosides; Superoxide anion; Verbascum xanthophoeniceum.