Abstract
In the present work, both 16S rRNA and pmoA gene-based PCR primers were employed successfully to study the diversity and distribution of n-damo bacteria in the surface and lower layer sediments at the coastal Mai Po wetland. The occurrence of n-damo bacteria in both the surface and subsurface sediments with high diversity was confirmed in this study. Unlike the two other known n-damo communities from coastal areas, the pmoA gene-amplified sequences in the present work clustered not only with some freshwater subclusters but also within three newly erected marine subclusters mostly, indicating the unique niche specificity of n-damo bacteria in this wetland. Results suggested vegetation affected the distribution and community structures of n-damo bacteria in the sediments and n-damo could coexist with sulfate-reducing methanotrophs in the coastal ecosystem. Community structures of the Mai Po n-damo bacteria based on 16S rRNA gene were different from those of either the freshwater or the marine. In contrast, structures of the Mai Po n-damo communities based on pmoA gene grouped with the marine ones and were clearly distinguished from the freshwater ones. The abundance of n-damo bacteria at this wetland was quantified using 16S rRNA gene PCR primers to be 2.65-6.71 × 10(5) copies/g dry sediment. Ammonium and nitrite strongly affected the community structures and distribution of n-damo bacteria in the coastal Mai Po wetland sediments.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anaerobiosis
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Bacteria / classification
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Bacteria / genetics
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Bacteria / isolation & purification*
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Bacteria / metabolism*
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Biodiversity*
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China
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DNA, Bacterial / genetics
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Fresh Water / microbiology
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Geologic Sediments / microbiology*
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Methane / metabolism*
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Molecular Sequence Data
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Nitrites / metabolism*
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Oxidation-Reduction
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Phylogeny
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S / genetics
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Soil Microbiology
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Wetlands
Substances
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Bacterial Proteins
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DNA, Bacterial
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Nitrites
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RNA, Ribosomal, 16S
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Methane
Associated data
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GENBANK/KJ718778
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GENBANK/KJ718779
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GENBANK/KJ718780
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