Point mutational inactivation of the retinoblastoma antioncogene

Science. 1989 Feb 17;243(4893):937-40. doi: 10.1126/science.2521957.


The retinoblastoma (Rb) antioncogene encodes a nuclear phosphoprotein, p105-Rb, that forms protein complexes with the adenovirus E1A and SV40 large T oncoproteins. A novel, aberrant Rb protein detected in J82 bladder carcinoma cells was not able to form a complex with E1A and was less stable than p105-Rb. By means of a rapid method for the detection of mutations in Rb mRNA, this defective Rb protein was observed to result from a single point mutation within a splice acceptor sequence in J82 genomic DNA. This mutation eliminates a single exon and 35 amino acids from its encoded protein product.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenovirus Early Proteins
  • Antigens, Polyomavirus Transforming
  • Base Sequence
  • DNA-Binding Proteins / metabolism
  • Eye Neoplasms / genetics*
  • Humans
  • Molecular Sequence Data
  • Mutation*
  • Oncogene Proteins, Viral / metabolism
  • Oncogenes*
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • Retinoblastoma / genetics*
  • Retinoblastoma Protein


  • Adenovirus Early Proteins
  • Antigens, Polyomavirus Transforming
  • DNA-Binding Proteins
  • Oncogene Proteins, Viral
  • Phosphoproteins
  • Retinoblastoma Protein