Profile of nucleosides and nucleotides in donkey's milk

Nucleosides Nucleotides Nucleic Acids. 2014;33(10):656-67. doi: 10.1080/15257770.2014.914219.


Nucleotides play a crucial role to cellular functions; they can be obtained from the diet or through the nucleotide salvage pathway, however, in particular situations (occurring mainly in newborns) the metabolic demand of nucleotides exceeds the capacity of their synthesis. These molecules, are receiving attention from a nutraceutical point of view because of their potential direct role in regulating metabolism and infant body condition. Donkey's milk may be considered a good replacer for cow's milk in feeding children with severe Ig-E mediated cow's milk protein allergy, due to its high similarity with human milk. In this study, the presence of cytidine, uridine, CMP, UMP, guanosine, and adenosine, involved in numerous biochemical and physiological activities, were detected for the first time through a RP-HPLC method.

Keywords: CMPA; Nucleosides; RP-HPLC; donkey's milk; infant nutrition; nucleotides.

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Diet
  • Equidae
  • Humans
  • Infant, Newborn
  • Milk / chemistry*
  • Milk Hypersensitivity
  • Milk Substitutes / chemistry*
  • Nucleosides / isolation & purification*
  • Nucleotides / isolation & purification*


  • Nucleosides
  • Nucleotides