Using amino-labeled nucleotide probes for simultaneous single molecule RNA-DNA FISH

PLoS One. 2014 Sep 16;9(9):e107425. doi: 10.1371/journal.pone.0107425. eCollection 2014.


Using amino-labeled oligonucleotide probes, we established a simple, robust and low-noise method for simultaneous detection of RNA and DNA by fluorescence in situ hybridization, a highly useful tool to study the large pool of long non-coding RNAs being identified in the current research. With probes either chemically or biologically synthesized, we demonstrate that the method can be applied to study a wide range of RNA and DNA targets at the single-cell and single-molecule level in cellular contexts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • DNA* / genetics
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Mice
  • Oligonucleotide Probes* / chemical synthesis
  • Oligonucleotide Probes* / chemistry
  • RNA* / genetics
  • Telomere / genetics


  • Oligonucleotide Probes
  • RNA
  • DNA