Phylogenetic position of the genus Cyrtostrombidium, with a description of Cyrtostrombidium paralongisomum nov. spec. and a redescription of Cyrtostrombidium longisomum Lynn & Gilron, 1993 (Protozoa, Ciliophora) based on live observation, protargol impregnation, and 18S rDNA sequences

J Eukaryot Microbiol. 2015 Mar-Apr;62(2):239-48. doi: 10.1111/jeu.12173. Epub 2014 Oct 16.

Abstract

We redescribe Cyrtostrombidium longisomum Lynn & Gilron, 1993, the type species of the genus Cyrtostrombidium, and describe the new species Cyrtostrombidium paralongisomum n. sp. using live observation, protargol staining and molecular data. The morphological characters of these two species are clearly distinct, i.e., dikinetid numbers in the girdle and ventral kineties; however, it is difficult to separate them by 18S rDNA sequences because they differ by only 8 bp, indicating that 18S rDNA sequences are insufficient for separating different species in the genus Cyrtostrombidium. We not only observed the position of the oral primordium in the genus Cyrtostrombidium but also observed a possibly homoplasious trait, a dorsal split in the girdle kinety, in (1) Apostrombidium, (2) Varistrombidium, and (3) Cyrtostrombidium/Williophrya. This partially supports the hypothesis of somatic ciliary pattern evolution recently put forth by Agatha and Strüder-Kypke.

Keywords: Ciliary pattern; Cyrtostrombidiidae; homoplasious; morphology; taxonomy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Ciliophora / classification*
  • Ciliophora / cytology
  • Ciliophora / genetics
  • Ciliophora / ultrastructure
  • DNA, Ribosomal / genetics
  • Genes, rRNA
  • Phylogeny*
  • RNA, Ribosomal, 18S / genetics
  • Seawater
  • Sequence Analysis, DNA
  • Silver Proteins

Substances

  • DNA, Ribosomal
  • RNA, Ribosomal, 18S
  • Silver Proteins
  • strong silver protein