RING finger proteins are involved in the progression of barrett esophagus to esophageal adenocarcinoma: a preliminary study

Gut Liver. 2014 Sep;8(5):487-94. doi: 10.5009/gnl13133. Epub 2014 Feb 24.


Background/aims: To investigate the differential expression of RING finger (RNF) proteins in Barrett esophagus (BE) and esophageal adenocarcinoma (EAC).

Methods: The differential expression of RNFs in normal esophagus (NE), BE, and EAC was screened using microarray assay. Real-time quantitative polymerase chain reaction (PCR), tissue micro-array assay, and Western blot analysis were independently performed to detect the mRNA and protein expression of screened RNFs.

Results: The expression of nine RNFs in the BE or EAC was 2-fold higher than those in NE. Among these proteins, the RNF32 and RNF121 expression in BE was 20.3-fold and 16.4-fold higher, respectively, than that in NE, and the expression of RNF24, RNF130, RNF141, RNF139, RNF11, RNF14, and RNF159 was upregulated more than 2-fold compared with NE. The expression of nine RNFs was not only upregulated in the EAC but was also positively related to the RNF expression in BE. The PCR results also indicated increased expression of these RNFs in BE and EAC compared to NE. Furthermore, the mRNA expression of all RNFs, except for RNF141 in EAC, was dramatically higher than those in the BE. Similar results were also obtained from the Western blot analysis.

Conclusions: A total of nine RNFs play critical roles in the progression of BE to EAC.

Keywords: Barrett esophagus; Esophageal adenocarcinoma; RING finger proteins; Tissue array analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / enzymology*
  • Adenocarcinoma / genetics
  • Adult
  • Aged
  • Barrett Esophagus / enzymology*
  • Barrett Esophagus / genetics
  • Carrier Proteins / genetics
  • DNA-Binding Proteins
  • Disease Progression
  • Esophageal Neoplasms / enzymology*
  • Esophageal Neoplasms / genetics
  • Female
  • Gene Expression Profiling
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics
  • Male
  • Middle Aged
  • Proteins / genetics
  • RING Finger Domains*
  • Receptors, Cell Surface / genetics
  • Ubiquitin-Protein Ligases / genetics
  • Ubiquitin-Protein Ligases / metabolism*


  • Carrier Proteins
  • DNA-Binding Proteins
  • Intracellular Signaling Peptides and Proteins
  • Proteins
  • RNF11 protein, human
  • RNF139 protein, human
  • RNF14 protein, human
  • RNF32 protein, human
  • Receptors, Cell Surface
  • RNF130 protein, human
  • RNF24 protein, human
  • Ubiquitin-Protein Ligases

Supplementary concepts

  • Adenocarcinoma Of Esophagus