Collagen peptide and vitamin C additively attenuate age-related skin atrophy in Sod1-deficient mice

Biosci Biotechnol Biochem. 2014;78(7):1212-20. doi: 10.1080/09168451.2014.915728. Epub 2014 May 28.


Age-related skin thinning is correlated with a decrease in the content of collagen in the skin. Accumulating evidence suggests that collagen peptide (CP) and vitamin C (VC) transcriptionally upregulate type I collagen in vivo. However, the additive effects of CP and VC on age-related skin changes remain unclear. We herein demonstrate that CP and a VC derivative additively corrected age-related skin thinning via reduced oxidative damage in superoxide dismutase 1 (Sod1)-deficient mice. Co-treatment with these compounds significantly normalized the altered gene expression of Col1a1, Has2, and Ci1, a proton-coupled oligopeptide transporter, in Sod1(-/-) skin. The in vitro analyses further revealed that collagen oligopeptide, a digestive product of ingested CP, significantly promoted the bioactivity of the VC derivative with respect to the migration and proliferation of Sod1(-/-) fibroblasts. These findings suggest that combined treatment with CP and VC is effective in cases of age-related skin pathology.

Keywords: collagen peptide; proton-coupled oligopeptide transporter; skin atrophy; superoxide dismutase; vitamin C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / pathology*
  • Animals
  • Ascorbic Acid / pharmacology*
  • Ascorbic Acid / therapeutic use
  • Atrophy / drug therapy
  • Collagen / chemistry*
  • Drug Synergism
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Fibroblasts / pathology
  • Male
  • Mice
  • Oxidative Stress / drug effects
  • Peptide Fragments / pharmacology*
  • Peptide Fragments / therapeutic use
  • Phenotype
  • Skin / drug effects*
  • Skin / metabolism
  • Skin / pathology*
  • Skin / physiopathology
  • Superoxide Dismutase / deficiency*
  • Superoxide Dismutase-1
  • Transcriptome / drug effects


  • Peptide Fragments
  • Collagen
  • Sod1 protein, mouse
  • Superoxide Dismutase
  • Superoxide Dismutase-1
  • Ascorbic Acid