Interferon and (2'-5')oligoadenylate enhance the expression of low affinity receptors for IgE (Fc epsilon R2/CD23) on the human monoblast cell line U937

Mol Immunol. 1989 Mar;26(3):241-7. doi: 10.1016/0161-5890(89)90077-1.

Abstract

The regulation of low affinity IgE receptor (Fc epsilon R2/CD23) expression on the human monoblast cell line U937 was examined by an anti-Fc epsilon R2/CD23 monoclonal antibody (H107) and the cDNA probe for Fc epsilon R2/CD23. Alpha interferon (IFN-alpha) and its intracellular mediator, (2'-5')oligoadenylate (2, 5-A), induced Fc epsilon R2/CD23 expression on U937 with no significant increase of the Fc epsilon R2/CD23 mRNA. PMA and IFN-gamma increased both surface Fc epsilon R2/CD23 expression and the Fc epsilon R2/CD23 mRNA levels. IFN-alpha effectively induced 2, 5-A synthetase activity in U937 cells, whereas IFN-gamma induced little. The results suggest that the mechanisms of enhancement of Fc epsilon R2/CD23 expression on U937 cells by IFN-alpha and IFN-gamma are different and that 2, 5-A may play an important role in the Fc epsilon R2/CD23 expression on U937 cells induced by IFN-alpha.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2',5'-Oligoadenylate Synthetase / metabolism
  • Adenine Nucleotides / pharmacology*
  • Antigens, Differentiation, B-Lymphocyte / analysis*
  • Blotting, Northern
  • Cell Line
  • Dexamethasone / pharmacology
  • Humans
  • Immunoglobulin E / metabolism*
  • Interferon Type I / pharmacology*
  • Oligoribonucleotides / pharmacology*
  • RNA, Messenger / analysis
  • Receptors, Fc / analysis*
  • Receptors, IgE

Substances

  • Adenine Nucleotides
  • Antigens, Differentiation, B-Lymphocyte
  • Interferon Type I
  • Oligoribonucleotides
  • RNA, Messenger
  • Receptors, Fc
  • Receptors, IgE
  • Immunoglobulin E
  • 2',5'-oligoadenylate
  • Dexamethasone
  • 2',5'-Oligoadenylate Synthetase