A role for 1α,25-dihydroxyvitamin d3 in the expression of circadian genes

J Biol Rhythms. 2014 Oct;29(5):384-8. doi: 10.1177/0748730414549239. Epub 2014 Sep 17.


The active form of vitamin D, 1α,25-(OH)2D3, has been associated with metabolism control, cell growth, differentiation, antiproliferation, apoptosis, and adaptive/innate immune responses, besides its functions in the integrity of bone and calcium homeostasis. The circadian rhythm regulates a variety of biological processes, many of them related to the functions associated with 1α,25-(OH)2D3. In the present study, we determine whether 1α,25-(OH)2D3 alters the expression of circadian genes in adipose-derived stem cells (ADSCs). The effect of 1α,25-(OH)2D3 on the expression of circadian genes BMAL1 and PER2 was measured by qPCR, over a 60-h period every 4 h, in serum shocked ADSCs, serum shocked ADSCs supplemented with 1α,25-(OH)2D3, and ADSCs under the presence of only 1α,25-(OH)2D3. The results showed that 1α,25-(OH)2D3 was able to synchronize circadian clock gene expression in ADSCs. The expression of circadian genes BMAL1 and PER2 in ADSCs that contained only 1α,25-(OH)2D3 has a profile similar to that found in the ADSCs synchronized by a serum shock. The results suggest an important role of 1α,25-(OH)2D3 in the regulation of the molecular clock.

Keywords: BMAL1; PER2; adipose-derived stem cells; serum shock; vitamin D.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ARNTL Transcription Factors / genetics
  • Circadian Rhythm / genetics*
  • Gene Expression
  • Humans
  • Period Circadian Proteins / genetics
  • Vitamin D / analogs & derivatives*
  • Vitamin D / genetics
  • Vitamin D / metabolism


  • ARNTL Transcription Factors
  • ARNTL protein, human
  • PER2 protein, human
  • Period Circadian Proteins
  • dihydroxy-vitamin D3
  • Vitamin D