Ubiquitin ligase CHIP suppresses cancer stem cell properties in a population of breast cancer cells

Biochem Biophys Res Commun. 2014 Oct 3;452(4):928-32. doi: 10.1016/j.bbrc.2014.09.011. Epub 2014 Sep 16.

Abstract

Cancer stem cells (CSCs) have several distinctive characteristics, including high metastatic potential, tumor-initiating potential, and properties that resemble normal stem cells such as self-renewal, differentiation, and drug efflux. Because of these characteristics, CSC is regarded to be responsible for cancer progression and patient prognosis. In our previous study, we showed that a ubiquitin E3 ligase carboxyl terminus of Hsc70-interacting protein (CHIP) suppressed breast cancer malignancy. Moreover, a recent clinical study reported that CHIP expression levels were associated with favorable prognostic parameters of patients with breast cancer. Here we show that CHIP suppresses CSC properties in a population of breast cancer cells. CHIP depletion resulted in an increased proportion of CSCs among breast cancers when using several assays to assess CSC properties. From our results, we propose that inhibition of CSC properties may be one of the functions of CHIP as a suppressor of cancer progression.

Keywords: Breast cancer; CHIP; Cancer stem cell.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology*
  • Cell Differentiation
  • Cell Proliferation
  • Female
  • Humans
  • MCF-7 Cells
  • Neoplastic Stem Cells / metabolism*
  • Neoplastic Stem Cells / pathology*
  • Ubiquitin-Protein Ligases / metabolism*

Substances

  • STUB1 protein, human
  • Ubiquitin-Protein Ligases