Negative pressure cavitation-microwave assisted preparation of extract of Pyrola incarnata Fisch. rich in hyperin, 2'-O-galloylhyperin and chimaphilin and evaluation of its antioxidant activity

Abstract

A novel and effective extraction method, namely negative pressure cavitation-microwave assisted extraction technique (NMAE), was developed for the preparation of extracts of Pyrola incarnata Fisch., which are rich in the main constituents hyperin, 2'-O-galloylhyperin and chimaphilin. Single factor experiments and Box-Behnken design (BBD) were combined with a response surface methodology to examine factors affecting extraction. Maximum extraction yields of hyperin, 2'-O-galloylhyperin and chimaphilin (1.339±0.029, 4.831±0.117 and 0.329±0.011mg/g, respectively) were achieved under the following optimised conditions: 700W microwave power, 50°C extraction temperature, 30:1mL/g liquid-solid ratio, -0.05MPa negative pressure, 55% ethanol concentration and 12min extraction time. First-order kinetics equation demonstrated that NMAE offered significant savings in extraction time, and enhancing extraction efficiency. Furthermore, NMAE extracts yielded excellent antioxidant activity (IC50 0.121mg/mL for DPPH 2.896mmol FeSO4/g DW FRAP).

Keywords: 2′-O-galloylhyperin (PubChem CID: 53399221); Antioxidant activity; Ascorbic acid (PubChem CID: 54670067); Chimaphilin (PubChem CID: 101211); DPPH (PubChem CID: 2735032); Ethanol (PubChem CID: 702); Formic acid (PubChem CID: 284); Hyperin (PubChem CID: 5281643); Mass transfer kinetics; Methanol (PubChem CID: 887); Negative pressure cavitation-microwave assisted extraction; Pyrola incarnata Fisch..