Rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells with antisense oligonucleotides

Tetsuro Hirose; Taro Mannen

doi:10.1007/978-1-4939-1369-5_13

Rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells with antisense oligonucleotides

Methods Mol Biol. 2015:1206:149-56. doi: 10.1007/978-1-4939-1369-5_13.

Authors

Tetsuro Hirose¹, Taro Mannen

Affiliation

¹ Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-ku, Sapporo, 060-0815, Japan, hirose@igm.hokudai.ac.jp.

PMID: 25240894
DOI: 10.1007/978-1-4939-1369-5_13

Abstract

A wide variety of noncoding RNAs are thought to play fundamental and regulatory functions in the mammalian cell nucleus, although their primary functions have yet to be elucidated. Here, we describe a method for the rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells using chemically modified antisense oligonucleotides, which may facilitate the functional assessment of nuclear ncRNAs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

HeLa Cells
Humans
Molecular Biology / methods*
Oligonucleotides, Antisense* / chemical synthesis
RNA, Untranslated*
Transfection / methods

Substances

Oligonucleotides, Antisense
RNA, Untranslated