Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9

FEBS Lett. 2014 Nov 3;588(21):3954-8. doi: 10.1016/j.febslet.2014.09.008. Epub 2014 Sep 19.


We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.

Keywords: Adenoviruses; CRISPR/Cas9; Gene editing; Liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Base Sequence
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
  • Gene Targeting / methods*
  • Humans
  • Liver / metabolism*
  • Male
  • Mice