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, 23 (1), 267-72

Sendai Virus C Protein Inhibits Lipopolysaccharide-Induced Nitric Oxide Production Through Impairing Interferon-β Signaling

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Sendai Virus C Protein Inhibits Lipopolysaccharide-Induced Nitric Oxide Production Through Impairing Interferon-β Signaling

Erdenezaya Odkhuu et al. Int Immunopharmacol.

Abstract

The effect of Sendai virus (SeV) C protein on lipopolysaccharide (LPS)-induced nitric oxide (NO) production was examined using RAW 264.7 macrophage cells. Infection of SeV inhibited LPS-induced NO production via downregulating the expression of an inducible NO synthase protein (iNOS). On the other hand, C gene-knockout 4C(-) SeV inhibited neither NO production nor iNOS expression. Wild type and 4C(-) SeV did not affect LPS-induced production of tumor necrosis factor-α and interleukin-6, and further LPS-induced activation of nuclear factor (NF)-κB and mitogen-activated protein kinases. Although wild type and 4C(-) SeV did not inhibit LPS-induced interferon (IFN)-β production, wild type SeV but not 4C(-) SeV inhibited the activation of STAT1/2 in the IFN-β signaling. SeV C protein inhibited LPS-induced iNOS expression and NO production. C protein inhibited the promotor activation of IFN-β and IFN-sensitive response element (ISRE) in response to LPS whereas the C mutant protein CF170S, which lacks the ability to block the STAT activation, did not inhibit it. Taken together, SeV C protein was suggested to inhibit LPS-induced NO production through impairing IFN-β signaling.

Keywords: C protein; Interferon-β; Lipopolysaccharide; Nitric oxide; Sendai virus.

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