[Use of adipose-derived stem cells in an experimental rotator cuff fracture animal model]

Rev Esp Cir Ortop Traumatol. Jan-Feb 2015;59(1):3-8. doi: 10.1016/j.recot.2014.07.008. Epub 2014 Sep 18.
[Article in Spanish]

Abstract

Aim: Rotator cuff repairs have shown a high level of re-ruptures. We hypothesized that the use of adipose-derived stem cells (ASC) could improve the biomechanical and histological properties of the repair.

Material and methods: Controlled experimental study conducted on 44 BDIX rats with section and repair of the supraspinatus tendon and randomization to one of three groups: group A, no intervention (control); group B, local applications of a fibrin sealant; and group C, application of the fibrin sealant with 2 x 10(6) ASC. At 4 and 8 weeks a biomechanical and histological analysis was performed.

Results: There were no differences in load-to-failure at 4 and 8 weeks between groups. The load-to-failure did increase between week 4 and week 8. Histologically the tendon-to bone union showed a disorganized fibrovascular tissue. Group C showed a different inflammatory pattern, with less presence of neutrophils and more presence of plasma cells.

Conclusion: The use of ASC does not improve the biomechanical or histological properties of the repair site. More studies are needed to improve techniques that enhance the healing site of the repair.

Keywords: Adipose-derived stem-cell; Cell therapy; Celula troncal derivada de lipoaspirado; Experimental; Manguito rotador; Repair; Reparación; Supraspinatus; Terapia celular.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Combined Modality Therapy
  • Fibrin Tissue Adhesive / therapeutic use
  • Mesenchymal Stem Cell Transplantation / methods*
  • Random Allocation
  • Rats
  • Rotator Cuff Injuries*
  • Subcutaneous Fat / cytology*
  • Tendon Injuries / therapy*
  • Treatment Outcome

Substances

  • Fibrin Tissue Adhesive