Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66

Braz J Microbiol. 2014 Aug 29;45(2):603-11. doi: 10.1590/s1517-83822014000200031. eCollection 2014.


Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p ≤ 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.

Keywords: Leclercia sp.; PGPR; bioinformatics; glucose dehydrogenase; pyrroloquinoline quinone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cluster Analysis
  • Computational Biology
  • Cytosol / chemistry
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae / isolation & purification
  • Enterobacteriaceae / physiology*
  • Glucose 1-Dehydrogenase / chemistry
  • Glucose 1-Dehydrogenase / genetics*
  • Models, Molecular
  • Molecular Sequence Data
  • Nerve Growth Factors*
  • Phaseolus / growth & development*
  • Phaseolus / microbiology*
  • Phosphorus / metabolism
  • Phylogeny
  • Plant Roots / growth & development
  • Plant Shoots / growth & development
  • Protein Conformation
  • Protein Structure, Tertiary
  • Quinones / analysis
  • Sequence Analysis, DNA
  • Sequence Homology


  • DNA, Bacterial
  • Nerve Growth Factors
  • Quinones
  • Phosphorus
  • Glucose 1-Dehydrogenase

Associated data

  • GENBANK/KC679993
  • GENBANK/KF156751