Dynamic changes in rat mesenteric lymph proteins following trauma using label-free mass spectrometry

Shock. 2014 Dec;42(6):509-17. doi: 10.1097/SHK.0000000000000259.


Early events triggered by posttrauma/hemorrhagic shock currently represent a leading cause of morbidity and mortality in these patients. The causative agents of these events have been associated with increased neutrophil priming secondary to shock-dependent alterations of mesenteric lymph. Previous studies have suggested that unknown soluble components of the postshock mesenteric lymph are main drivers of these events. In the present study, we applied a label-free proteomics approach to further delve into the early proteome changes of the mesenteric lymph in response to hemorrhagic shock. Time-course analyses were performed by sampling the lymph every 30 min after shock up until 3 h (the time window within which a climax in neutrophil priming was observed). There are novel, transient early post-hemorrhagic shock alterations to the proteome and previously undocumented postshock protein alterations. These results underlie the triggering of coagulation and proinflammatory responses secondary to trauma/hemorrhagic shock, metabolic deregulation and apoptosis, and alterations to proteases/antiproteases homeostasis, which are suggestive of the potential implication of extracellular matrix proteases in priming neutrophil activation. Finally, there is a likely correlation between early postshock mesenteric lymph-mediated neutrophil priming and proteomics changes, above all protease/antiproteases impaired homeostasis (especially of serine proteases and metalloproteases).

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Acute-Phase Proteins / chemistry
  • Animals
  • Apoptosis
  • Blood Coagulation
  • Gelsolin / chemistry
  • Inflammation
  • Kallikreins / chemistry
  • Lymph / metabolism*
  • Male
  • Mass Spectrometry / methods*
  • Mesentery / metabolism*
  • Neutrophil Activation
  • Neutrophils / metabolism
  • Oxygen Consumption
  • Peptide Hydrolases / chemistry
  • Proteomics / methods*
  • Rats
  • Rats, Sprague-Dawley
  • Serine Proteases / chemistry
  • Shock, Hemorrhagic / metabolism
  • Time Factors
  • Wounds and Injuries / metabolism


  • Acute-Phase Proteins
  • Gelsolin
  • Peptide Hydrolases
  • Serine Proteases
  • Kallikreins