UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo

Methods Mol Biol. 2015;1189:219-35. doi: 10.1007/978-1-4939-1164-6_15.

Abstract

Mechanically coupled cells can generate forces driving cell and tissue morphogenesis during development. Visualization and measuring of these forces is of major importance to better understand the complexity of the biomechanic processes that shape cells and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively assess mechanical tension in different tissues of the developing zebrafish and in cultures of primary germ layer progenitor cells ex vivo.

MeSH terms

  • Actomyosin / metabolism
  • Animals
  • Biomechanical Phenomena
  • Cells, Cultured
  • Embryo, Nonmammalian / cytology*
  • Embryo, Nonmammalian / physiology*
  • Laser Therapy / methods*
  • Mesoderm / cytology
  • Stem Cells / cytology
  • Ultraviolet Rays*
  • Zebrafish / embryology*

Substances

  • Actomyosin