Response of intracellular Ca2+ transients in cultured vascular smooth muscle cells to angiotensin II, vasopressin, acetylcholine and atrionatriuretic peptide

Jpn Heart J. 1989 Jan;30(1):77-83. doi: 10.1536/ihj.30.77.


Modulation of intracellular Ca2+ concentration [( Ca2+]i) is a signal for the contraction of vascular smooth muscle cells responding to vasoreactive substances. We prepared confluently cultured smooth muscle cells from rat aorta, loaded them with Ca2+ sensitive fluorescent dye, fura-2, and measured the [Ca2+]i transient by microscopic spectrofluorometry. The [Ca2+]i was distributed heterogeneously in cytosol. Angiotensin II (10 nM) transiently doubled the [Ca2+]i. It was also increased by arginine-vasopressin (10 nM), even after stimulation by angiotensin II was saturated. In contrast, acetylcholine (10 microM) or rat atrionatriuretic peptide (10 nM) did not change the [Ca2+]i in the same detecting field of the same cell, contradicting previous reports.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / pharmacology
  • Angiotensin II / pharmacology
  • Animals
  • Arginine Vasopressin / pharmacology
  • Atrial Natriuretic Factor / pharmacology
  • Benzofurans
  • Calcium / metabolism*
  • Cells, Cultured
  • Cytosol / metabolism
  • Fluorometry
  • Fura-2
  • Intracellular Membranes / metabolism*
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Spectrophotometry
  • Stimulation, Chemical


  • Benzofurans
  • Angiotensin II
  • Arginine Vasopressin
  • Atrial Natriuretic Factor
  • Acetylcholine
  • Calcium
  • Fura-2