[Expression of IL-35, Epstein-Barr Virus-Induced Gene 3 mRNA and IL-12A mRNA in Peripheral Blood of Patients With Allergic Rhinitis]

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 Jul;28(13):952-4.
[Article in Chinese]


Objective: To explore the expression of IL-35, Epstein-Barr virus-induced gene 3 (EBI3) mRNA and IL-12A mRNA in peripheral blood of patients with allergic rhinitis and its significance.

Method: Peripheral blood were collected from patients with allergic rhinitis (46 cases) and healthy human controls(30 cases). The level of IL-35 in serum was measured by enzyme-linked immunosorbent assay. The subunit EB13 and IL-12A of IL-35 mRNA expressions in peripheral blood mononuclear cell(PBMC) were detected by SYBR Green real-time quantitative PCR.

Result: IL-35 level in AR group (251.22 +/- 46.27) ng/L was significantly lower compared with that in the normal control group (382.17 +/- 25.41) ng/L, (P < 0.01). The mRNA expression level of EBI3 in the patients with allergic rhinitis was significantly lower than that in the normal control group (P < 0.01), AR group EB13 mRNA level was about half of that in the normal control group. But the mRNA expression level of IL-12A in the patients with allergic rhinitis has not significant difference with that in the normal control group (P > 0. 05).

Conclusion: The decrease of IL-35 and EBI3 mRNA in AR,indicated that IL-35 and EBl3 mRNA may play an important role in allergic rhinitis.

MeSH terms

  • Adolescent
  • Adult
  • Case-Control Studies
  • Child
  • Female
  • Humans
  • Interleukin-12 Subunit p35 / blood*
  • Interleukins / blood*
  • Male
  • Middle Aged
  • Minor Histocompatibility Antigens
  • Rhinitis, Allergic / blood*
  • Young Adult


  • EBI3 protein, human
  • IL12A protein, human
  • Interleukin-12 Subunit p35
  • Interleukins
  • Minor Histocompatibility Antigens
  • interleukin-35, human