Additive effects of two quantitative trait loci that confer Rhopalosiphum maidis (corn leaf aphid) resistance in maize inbred line Mo17

J Exp Bot. 2015 Feb;66(2):571-8. doi: 10.1093/jxb/eru379. Epub 2014 Sep 23.

Abstract

Plants show considerable within-species variation in their resistance to insect herbivores. In the case of Zea mays (cultivated maize), Rhopalosiphum maidis (corn leaf aphids) produce approximately twenty times more progeny on inbred line B73 than on inbred line Mo17. Genetic mapping of this difference in maize aphid resistance identified quantitative trait loci (QTL) on chromosomes 4 and 6, with the Mo17 allele reducing aphid reproduction in each case. The chromosome 4 QTL mapping interval includes several genes involved in the biosynthesis of DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one), a maize defensive metabolite that also is required for callose accumulation in response to aphid feeding. Consistent with the known association of callose with plant defence against aphids, R. maidis reproduction on B73×Mo17 recombinant inbred lines was negatively correlated with both DIMBOA content and callose formation. Further genetic mapping, as well as experiments with near-isogenic lines, confirmed that the Mo17 allele causes increased DIMBOA accumulation relative to the B73 allele. The chromosome 6 aphid resistance QTL functions independently of DIMBOA accumulation and has an effect that is additive to that of the chromosome 4 QTL. Thus, at least two separate defence mechanisms account for the higher level of R. maidis resistance in Mo17 compared with B73.

Keywords: DIMBOA; Rhopalosiphum maidis; aphid; benzoxazinoid; callose; maize; quantitative trait..

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Aphids / physiology*
  • Benzoxazines / chemistry
  • Benzoxazines / metabolism
  • Chromosome Mapping
  • Disease Resistance / genetics*
  • Glucans / metabolism
  • Inbreeding*
  • Models, Biological
  • Plant Diseases / genetics
  • Plant Diseases / immunology
  • Plant Diseases / parasitology*
  • Quantitative Trait Loci / genetics*
  • Reproduction
  • Zea mays / genetics*
  • Zea mays / immunology
  • Zea mays / parasitology*

Substances

  • Benzoxazines
  • Glucans
  • callose
  • 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one