Background: Although the incidence of gastric cancer is declining during the last half century, this cancer still is the second morbid cancer in the world after lung cancer. The incidence of gastric cancer is 26 per 100,000 in Iran. This study evaluated the effect of Alpinia galangal on AGS cells (human gastric adenocarcinoma epithelial cell line) and L929 cells (as a standard cell line originated from mouse fibroblast cells).
Methods: After culturing the cells in Roswell Park Memorial Institute (RPMI) medium, the cells were incubated with different doses of Alpinia galangal (0 (control), 125, 250, 500, 750 and 1000 µg/ml) in 24, 48 and 72 hour periods and then, cells viability were assessed using MTT based cell proliferation assay.
Results: After 24 hours, the percentage of living AGS cells compared to the control group showed no significant decrease at the concentrations of 125 and 250µg/ml. But in the rest concentrations were significant (p<0.05). Only, the percentage of surviving L929 cells at concentration of 125µg/ml of the extract was not significant, but these percentages in the other concentrations were significant. After 48 and 72h incubation, in the last three extract concentrations, the percentage of living AGS and L929 cells significantly decreased compared to control cells (p<0.05).
Conclusion: We have demonstrated, using cell culture model, anti-proliferative effect of aqueous extract of Alpinia galangal on human gastric tumor (AGS) and L929 cell lines. This effect was prominent in high concentrations.
Keywords: AGS; Alpinia galangal; L929; MTT.