miR-125b-1-3p inhibits trophoblast cell invasion by targeting sphingosine-1-phosphate receptor 1 in preeclampsia

Biochem Biophys Res Commun. 2014 Oct 10;453(1):57-63. doi: 10.1016/j.bbrc.2014.09.059. Epub 2014 Sep 22.


Preeclampsia (PE) is the leading cause of maternal and perinatal mortality and morbidity. Understanding the molecular mechanisms underlying placentation facilitates the development of better intervention of this disease. MicroRNAs are strongly implicated in the pathogenesis of this syndrome. In current study, we found that miR-125b-1-3p was elevated in placentas derived from preeclampsia patients. Transfection of miR-125b-1-3p mimics significantly inhibited the invasiveness of human trophoblast cells, whereas miR-125b-1-3p inhibitor enhanced trophoblast cell invasion. Luciferase assays identified that S1PR1 was a novel direct target of miR-125b-1-3p in the placenta. Overexpression of S1PR1 could reverse the inhibitory effect of miR-125b-1-3p on the invasion of trophoblast cells. These findings suggested that abnormal expression of miR-125b-1-3p might contribute to the pathogenesis of preeclampsia.

Keywords: Invasion; Preeclampsia; S1PR1; Trophoblast; miR-125b-1-3p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Case-Control Studies
  • Cell Line
  • Cell Movement / genetics
  • Cell Movement / physiology
  • Female
  • Gene Expression
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • Placenta / metabolism
  • Placenta / pathology
  • Placentation / genetics
  • Placentation / physiology
  • Pre-Eclampsia / genetics*
  • Pre-Eclampsia / metabolism*
  • Pre-Eclampsia / pathology
  • Pregnancy
  • Receptors, Lysosphingolipid / genetics
  • Receptors, Lysosphingolipid / metabolism*
  • Sphingosine-1-Phosphate Receptors
  • Trophoblasts / metabolism*
  • Trophoblasts / pathology*


  • MIRN125 microRNA, human
  • MicroRNAs
  • Receptors, Lysosphingolipid
  • S1PR1 protein, human
  • Sphingosine-1-Phosphate Receptors