Doxycycline enhances survival and self-renewal of human pluripotent stem cells

Mi-Yoon Chang; Yong-Hee Rhee; Sang-Hoon Yi; Su-Jae Lee; Rae-Kwon Kim; Hyongbum Kim; Chang-Hwan Park; Sang-Hun Lee

doi:10.1016/j.stemcr.2014.06.013

Doxycycline enhances survival and self-renewal of human pluripotent stem cells

Stem Cell Reports. 2014 Aug 12;3(2):353-64. doi: 10.1016/j.stemcr.2014.06.013. Epub 2014 Jul 24.

Authors

Mi-Yoon Chang¹, Yong-Hee Rhee², Sang-Hoon Yi¹, Su-Jae Lee³, Rae-Kwon Kim³, Hyongbum Kim⁴, Chang-Hwan Park⁴, Sang-Hun Lee⁵

Affiliations

¹ Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea.
² Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Korea.
³ Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, Korea.
⁴ Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Korea.
⁵ Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul 133-791, Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul 133-791, Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 133-791, Korea. Electronic address: leesh@hanyang.ac.kr.

Abstract

We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These doxycycline effects are not associated with its antibacterial action, but mediated by direct activation of a PI3K-AKT intracellular signal. These findings indicate doxycycline as a useful supplement for stem cell cultures, facilitating their growth and maintenance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Cell Differentiation / drug effects
Cell Proliferation / drug effects*
Cell Survival / drug effects
Cells, Cultured
Doxycycline / pharmacology*
Embryonic Stem Cells / cytology
Humans
Karyotyping
Neural Stem Cells / cytology
Neurons / cytology
Phosphatidylinositol 3-Kinases / metabolism
Pluripotent Stem Cells / cytology
Pluripotent Stem Cells / drug effects*
Pluripotent Stem Cells / metabolism
Proto-Oncogene Proteins c-akt / metabolism
Signal Transduction / drug effects

Substances

Anti-Bacterial Agents
Phosphatidylinositol 3-Kinases
Proto-Oncogene Proteins c-akt
Doxycycline