Association Between Chlorinated Pesticides in the Serum of Prepubertal Russian Boys and Longitudinal Biomarkers of Metabolic Function

Am J Epidemiol. 2014 Nov 1;180(9):909-19. doi: 10.1093/aje/kwu212. Epub 2014 Sep 25.

Abstract

Organochlorine pesticides (OCPs) have been linked to adult metabolic disorders; however, few studies have examined these associations in childhood. We prospectively evaluated the associations of baseline serum OCPs (hexachlorobenzene, β-hexachlorocyclohexane, and p,p'-dichlorodiphenyldichloroethylene) in Russian boys with subsequent repeated measurements of serum glucose, insulin, lipids, leptin, and calculated homeostatic model assessment of insulin resistance (IR). During 2003-2005, we enrolled 499 boys aged 8-9 years in a prospective cohort; 318 had baseline serum OCPs and serum biomarkers measured at ages 10-13 years. Multivariable generalized estimating equation and mediation regression models were used to examine associations and direct and indirect (via body mass index (BMI) (weight (kg)/height (m)(2))) effects of prepubertal OCP tertiles and quintiles with biomarkers. In multivariable models, higher p,p'-dichlorodiphenyldichloroethylene (quintile 5 vs. quintile 1) was associated with lower leptin, with relative mean decreases of 61.8% (95% confidence interval: 48.4%, 71.7%) in models unadjusted for BMI and 22.2% (95% confidence interval: 7.1%, 34.9%) in models adjusted for BMI; the direct effect of p,p'-dichlorodiphenyldichloroethylene on leptin accounted for 27% of the total effect. IR prevalence was 6.6% at ages 12-13 years. Higher hexachlorobenzene (tertile 3 vs. tertile 1) was associated with higher odds of IR in models adjusted for BMI (odds ratio = 4.37, 95% confidence interval: 1.44, 13.28). These results suggest that childhood OCPs may be associated with IR and lower leptin.

Keywords: children; insulin resistance; leptin; metabolism; pesticides.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adolescent
  • Biomarkers / blood
  • Blood Glucose / analysis
  • Child
  • Cholesterol / blood
  • Humans
  • Hydrocarbons, Chlorinated / blood*
  • Insulin / blood
  • Insulin Resistance*
  • Leptin / blood
  • Male
  • Metabolic Syndrome / blood*
  • Pesticides / blood*
  • Prospective Studies
  • Regression Analysis
  • Triglycerides / blood

Substances

  • Biomarkers
  • Blood Glucose
  • Hydrocarbons, Chlorinated
  • Insulin
  • Leptin
  • Pesticides
  • Triglycerides
  • Cholesterol