A novel tri-component scheme for classifying neuronal discharge patterns

J Neurosci Methods. 2015 Jan 15;239:148-61. doi: 10.1016/j.jneumeth.2014.09.015. Epub 2014 Sep 23.

Abstract

Background: Neuronal discharge patterns can be described by three principle patterns, namely, regular, irregular, and bursty.

New method: Available discrimination metrics, including global ISI metrics (e.g., coefficient of variation (CV), asymmetric index), local variables (CV2, CV for a sequence of two ISIs; IR, difference of log of two adjacent ISIs; LVr, local variation with refractory period), Fano factor (FF) and Allan factor (AF), and three new burst metrics, 'burst percentage' (BP), 'burst tendency' (BT) and 'burst entropy' (BE), were extensively tested on representative simulated spike trains. Upon verifying that individual metrics could not by themselves reliably classify the diverse simulation patterns, a novel tri-component classification algorithm was developed. Inadequate metrics were rejected and the remaining selected metrics were grouped and weighted using multiple metric optimization to form three proxy metrics: 'regularity' (combining local variables), 'burstiness' (combining BP, BT and BE), and 'corruption' (combining FF and AF).

Results: The accuracy of the proxy metrics was verified on a large set of neuronal spike trains extracellularly recorded from multiple regions of the brain in unsedated normal and dystonic rats. Cross-validation of the tri-component classifier against meticulous subjective classification of these data demonstrated an agreement of 95.9%, with a high discriminatory power of 2.6.

Comparison with existing methods: The tri-component classifier was demonstrated to well outperform individual metrics on all aspects of pattern and corruption discrimination.

Conclusions: The tri-component classifier provides a novel, reliable algorithm to differentiate highly diverse neuronal discharge patterns and discriminate natural or erroneous corruption in the signal.

Keywords: Burstiness; Inter-spike interval; Multiple metric optimization; Non-stationarity; Pattern discrimination; Poisson distribution; Spike train; k Mean clustering.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Action Potentials / physiology*
  • Algorithms*
  • Animals
  • Brain / cytology
  • Models, Neurological*
  • Neurons / classification*
  • Neurons / physiology*
  • Principal Component Analysis
  • ROC Curve
  • Rats