Loss of MBNL Leads to Disruption of Developmentally Regulated Alternative Polyadenylation in RNA-mediated Disease

Mol Cell. 2014 Oct 23;56(2):311-322. doi: 10.1016/j.molcel.2014.08.027. Epub 2014 Sep 25.

Abstract

Inhibition of muscleblind-like (MBNL) activity due to sequestration by microsatellite expansion RNAs is a major pathogenic event in the RNA-mediated disease myotonic dystrophy (DM). Although MBNL1 and MBNL2 bind to nascent transcripts to regulate alternative splicing during muscle and brain development, another major binding site for the MBNL protein family is the 3' untranslated region of target RNAs. Here, we report that depletion of Mbnl proteins in mouse embryo fibroblasts leads to misregulation of thousands of alternative polyadenylation events. HITS-CLIP and minigene reporter analyses indicate that these polyadenylation switches are a direct consequence of MBNL binding to target RNAs. Misregulated alternative polyadenylation also occurs in skeletal muscle in a mouse polyCUG model and human DM, resulting in the persistence of neonatal polyadenylation patterns. These findings reveal an additional developmental function for MBNL proteins and demonstrate that DM is characterized by misregulation of pre-mRNA processing at multiple levels.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Alternative Splicing / genetics*
  • Animals
  • Binding Sites / genetics
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cells, Cultured
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Developmental
  • Humans
  • Mice
  • Microsatellite Repeats / genetics
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / metabolism
  • Myotonic Dystrophy / genetics
  • Polyadenylation / genetics*
  • Protein Binding
  • RNA Interference
  • RNA Precursors / genetics
  • RNA Processing, Post-Transcriptional / genetics
  • RNA, Messenger / metabolism
  • RNA, Small Interfering
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism

Substances

  • 3' Untranslated Regions
  • Carrier Proteins
  • DNA-Binding Proteins
  • MBNL3 protein, mouse
  • Mbnl1 protein, mouse
  • Mbnl2 protein, mouse
  • RNA Precursors
  • RNA, Messenger
  • RNA, Small Interfering
  • RNA-Binding Proteins

Associated data

  • GEO/GSE60487