Separation and purification of thymopentin with molecular imprinting membrane by solid phase extraction disks

Chaoli Wang; Xiaoling Hu; Ping Guan; Danfeng Wu; Liwei Qian; Ji Li; Renyuan Song

doi:10.1016/j.jpba.2014.07.016

Separation and purification of thymopentin with molecular imprinting membrane by solid phase extraction disks

J Pharm Biomed Anal. 2015 Jan:102:137-43. doi: 10.1016/j.jpba.2014.07.016. Epub 2014 Aug 1.

Authors

Chaoli Wang¹, Xiaoling Hu², Ping Guan², Danfeng Wu², Liwei Qian², Ji Li², Renyuan Song²

Affiliations

¹ College of Science, Northwestern Polytechnical University, Xi'an 710072, China. Electronic address: wcl1982@mail.nwpu.edu.cn.
² College of Science, Northwestern Polytechnical University, Xi'an 710072, China.

PMID: 25265188
DOI: 10.1016/j.jpba.2014.07.016

Abstract

The synthesis and performance of molecularly imprinted membranes (MIMs) as a solid phase extraction packing materials for the separation and purification of thymopentin from crude samples was described. In order to increase structural selectivity and imprinting efficiency, surface-initiated ATRP and ionic liquid (1-vinyl-3-ethyl acetate imidazolium chloride) were used to prepare molecularly imprinting membranes. The results demonstrated that solid phase extraction disks stuffed by MIMs with ionic liquids as functional monomer demonstrated high isolation and purification of performance to the thymopentin. The molecular recognition of thymopentin was analyzed by using molecular modeling software.

Keywords: Ionic liquids; Molecular simulation; Molecularly imprinting membrane; Solid phase extraction disk; Thymopentin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Ionic Liquids / chemical synthesis
Membranes, Artificial*
Models, Molecular
Molecular Imprinting
Solid Phase Extraction
Thymopentin / analysis*

Substances

Ionic Liquids
Membranes, Artificial
Thymopentin