Fabp4-CreER lineage tracing reveals two distinctive coronary vascular populations

J Cell Mol Med. 2014 Nov;18(11):2152-6. doi: 10.1111/jcmm.12415. Epub 2014 Sep 30.


Over the last two decades, genetic lineage tracing has allowed for the elucidation of the cellular origins and fates during both embryogenesis and in pathological settings in adults. Recent lineage tracing studies using Apln-CreER tool indicated that a large number of post-natal coronary vessels do not form from pre-existing vessels. Instead, they form de novo after birth, which represents a coronary vascular population (CVP) distinct from the pre-existing one. Herein, we present new coronary vasculature lineage tracing results using a novel tool, Fabp4-CreER. Our results confirm the distinct existence of two unique CVPs. The 1(st) CVP, which is labelled by Fabp4-CreER, arises through angiogenic sprouting of pre-existing vessels established during early embryogenesis. The 2(nd) CVP is not labelled by Fabp4, suggesting that these vessels form de novo, rather than through expansion of the 1(st) CVP. These results support the de novo formation of vessels in the post-natal heart, which has implications for studies in cardiovascular disease and heart regeneration.

Keywords: angiogenesis; coronary artery diseases; coronary vessel; heart regeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Cardiovascular Diseases / genetics*
  • Cardiovascular Diseases / pathology
  • Cell Lineage / genetics
  • Coronary Vessels / cytology
  • Coronary Vessels / growth & development
  • Coronary Vessels / metabolism
  • Embryonic Development*
  • Fatty Acid-Binding Proteins / biosynthesis*
  • Fatty Acid-Binding Proteins / genetics
  • Gene Expression Regulation, Developmental
  • Heart / growth & development*
  • Heart / physiopathology
  • Humans
  • Integrases / genetics
  • Mice
  • Morphogenesis / genetics
  • Neovascularization, Physiologic / genetics
  • Regeneration / genetics


  • FABP4 protein, human
  • Fatty Acid-Binding Proteins
  • Cre recombinase
  • Integrases