Saline storage of aerobic granules and subsequent reactivation

Chunli Wan; Duu-Jong Lee; Xue Yang; Yayi Wang; Lin Lin

doi:10.1016/j.biortech.2014.08.103

Saline storage of aerobic granules and subsequent reactivation

Bioresour Technol. 2014 Nov:172:418-422. doi: 10.1016/j.biortech.2014.08.103. Epub 2014 Aug 30.

Authors

Chunli Wan¹, Duu-Jong Lee², Xue Yang³, Yayi Wang⁴, Lin Lin³

Affiliations

¹ Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
² Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan. Electronic address: djleetw@yahoo.com.tw.
³ Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
⁴ State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.

PMID: 25270079
DOI: 10.1016/j.biortech.2014.08.103

Abstract

Loss of structural stability and bioactivity during long-term storage and operation is primary challenge to field applications of aerobic granular processes. This study for the first time stored aerobic granules in 5%w/w NaCl solution at 4°C for 187d. The stored granules were then successfully reactivated and used for 85d in sequencing batch reactors (SBR) and continuous-flow reactors (CFR) at varying levels of chemical oxygen demand (COD). High-throughput sequencing results reveal that Thauera sp., Paracoccus sp., and Nitrosomonas sp. were the predominant in the stored aerobic granules, and Pseudoxanthomonas sp. accumulated during the reactivation process. Saline storage, in which cells are in an unculturable state by saline stress, is a promising storage process for aerobic granules.

Keywords: Aerobic granules; High throughput sequencing; Reactivation; SOUR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria, Aerobic / cytology
Bacteria, Aerobic / isolation & purification*
Bacteria, Aerobic / physiology*
Batch Cell Culture Techniques / methods*
Bioreactors / microbiology*
Cell Proliferation / physiology
Cell Survival / physiology
Sodium Chloride / chemistry*

Substances

Sodium Chloride