The Transcription Factor Con7-1 Is a Master Regulator of Morphogenesis and Virulence in Fusarium oxysporum

Mol Plant Microbe Interact. 2015 Jan;28(1):55-68. doi: 10.1094/MPMI-07-14-0205-R.

Abstract

Previous studies have demonstrated the essential role of morphogenetic regulation in Fusarium oxysporum pathogenesis, including processes such as cell-wall biogenesis, cell division, and differentiation of infection-like structures. We identified three F. oxysporum genes encoding predicted transcription factors showing significant identities to Magnaporthe oryzae Con7p, Con7-1, plus two identical copies of Con7-2. Targeted deletion of con7-1 produced nonpathogenic mutants with altered morphogenesis, including defects in cell wall structure, polar growth, hyphal branching, and conidiation. By contrast, simultaneous inactivation of both con7-2 copies caused no detectable defects in the resulting mutants. Comparative microarray-based gene expression analysis indicated that Con7-1 modulates the expression of a large number of genes involved in different biological functions, including host-pathogen interactions, morphogenesis and development, signal perception and transduction, transcriptional regulation, and primary and secondary metabolism. Taken together, our results point to Con7-1 as general regulator of morphogenesis and virulence in F. oxysporum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Animals
  • Cell Wall / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Fusarium / genetics*
  • Fusarium / growth & development
  • Fusarium / pathogenicity
  • Fusarium / ultrastructure
  • Gene Expression Profiling
  • Gene Expression Regulation, Fungal / genetics*
  • Glucose / metabolism
  • Host-Pathogen Interactions
  • Hyphae
  • Larva
  • Lycopersicon esculentum / microbiology*
  • Magnaporthe / genetics
  • Moths
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • Plant Diseases / microbiology*
  • Plant Roots / microbiology
  • Sequence Deletion
  • Spores, Fungal
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Virulence

Substances

  • Fungal Proteins
  • Transcription Factors
  • Glucose

Associated data

  • GEO/GSE59311